Brook trout are a recreationally and ecologically important native fish species across the Eastern United States. They are sensitive to water quality and temperature making them strong indicators of stream health. However, their populations have declined due to land use changes, habitat fragmentation and warming water temperature. This project aims to enhance brook trout habitat conservation and restoration by using cutting-edge data and tools. The Chesapeake Conservancy will develop a web-based decision support tool that helps state and federal agencies identify the most impactful stream reaches for habitat conservation and restoration. The tool will use newly developed high-resolution stream data known as Hyper Resolution Hydrography Data, which identifies more than twice as many stream miles compared to the traditional National Hydrography Dataset (NHD), especially headwater streams critical to brook trout. The project will evaluate how landscape features such as forest cover or agriculture affect brook trout habitat and identify which streams offer the greatest opportunity for habitat improvement. These analyses will be used to create an interactive publicly available mapping application. This tool will allow agencies to explore what-if restoration scenarios and target their efforts for maximum benefit. Additionally, the project will compare conservation opportunities identified using traditional NHD data versus those using the more complete Hyper Resolution dataset. By doing so, it will demonstrate the added value of using LiDAR derived data in fisheries management. The project will also provide guidance for states interested in replicating this high resolution data approach outside the Chesapeake Bay watershed. The project directly supports the Association of Fish and Wildlife Agencies’ strategic priorities of using emerging technologies, e.g., LiDAR and improving landscape-level habitat connectivity. It will benefit multiple states within the Northeast Association of Fish and Wildlife Agencies (NEAFWA) and U.S. Fish and Wildlife Service Region 5. Results will help these states efficiently allocate conservation resources, improve regional coordination and ultimately strengthen brook trout populations. Deliverables include the prioritization tool geospatial databases training materials and a final report summarizing findings and differences in restoration opportunities with and without Hyper Resolution data. Outreach will include training webinars and presentations at regional conferences.